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Pharmacia single path monitor UV1 control/optical units
Pharmacia Single Path Monitor UV-1 Control and Optical Units.
Excellent cosmetic condition. Power up, appear to be functioning properly, but cannot be fully tested.
The UV-1 is a fixed wavelength UV monitor, consisting of a control unit and an optical unit that can be positioned up to 10 meters apart. A mercury lamp is the stable light source, furthermore a built-in reference cell eliminates baseline drift. The output signal can be recorded in either AU or %T. Sensitivity range between 0.01 and 2 AUFS, or (0-100%T).
The UV-1 offers a range of three detection wavelengths: 254, 280 and 405 nm. In addition, five flow cells are available for different applications in Standard Chromatography, FPLC and Industrial applications.
GE Healthcare LKB Pharmacia Monitor UV-1 consists of an optical unit containing the flow cell, lamp, filter assemblies and preamplifiers, and a control unit containing the signal processing circuits. The two units are connected via a multi-core cable hardwired from the control unit to the optical unit. Connection to the recorder and the mains supply is made via the control unit.
The single path Monitor UV-1 is a dual beam instrument with a sample cell and a reference cell. Optical path lengths are 10 mm, 3 mm, or 2 mm depending upon the flow cell chosen.
Light from the lamp passes through an aperture (254 nm, 405 nm) or a fluorescence converter (280 nm) and through the reference cell and sample cell. The light output from each cell passes through an interference filter and falls onto a solid state photo-detector whose output is a linear function of the light intensity.
The photocurrent from each detector is amplified in a pre-amplifier before passing to the signal processing circuitry in the control unit.
If transmission is to be monitored, the signal passes directly to the low pass filter. If AU is to be monitored, the reference cell light intensity (IR) is compared with the sample cell intensity (IS) to form log (IR/IS) in a logarithmic circuit before passing to the low-pass filter. The signal finally passes to the range selector before being presented to the output terminal.
