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megabase 1000 dna sequencer dna analyzer genotyper
MegaBACE 1000 DNA Analysis System is a high-throughput, fluorescence-based DNA system utilizing capillary electrophoresis with up to 96 capillaries operating in parallel. The system performs sample injection, gel matrix replacement, DNA separation, detection, and data analysis, offering unparralleled flexibility.
* Capillary electrophoresis instrument
* Up to 6 arrays of 16 capillaries/array for a total of 96 capillaries
* Separation matrix for DNA analysis
* Reagents for DNA sequencing and genotyping
* Instrument control and data analysis software
* Computer workstation and monitor for instrument operation and data analysis
The capillaries are supplied in pre-assembled arrays of 16 capillaries. 16, 32, 48, 64, 80 or 96 capillaries can be used during a run.
MegaBACE Long Read Matrix is optimized for both sequencing and genotyping applications. This saves time when switching between applications, and results in less waste.
MegaBACE Long Read Matrix can produce DNA sequences of 700 bases or resolve multiplexed fragments in excess of 900 bases. The gel matrix is replaceable, and each pack includes 96 pre-dispensed tubes plus running buffer.
All MegaBACE DNA sequencing reagent kits offer unparalleled performance on a broad range of templates:
* DYEnamic ET Dye Terminators or DYEnamic ET Primers
* DYEnamic ET Primer Reagents
To complement instrument functionality, we offer a range of MegaBACE ET Size Standards for a variety of applications. These include MegaBACE ET400-R, ET550-R, and ET900-R.
For SNP validation, MegaBACE SNuPe Genotyping Kit contains everything needed to perform a robust single-base extension reaction of PCR products in a single-tube format.
The following software tools are available:
* Instrument Control Manager is used for instrument set-up and sample entry.
* Sequence Analyzer processes large amounts of data without manual intervention.
* MegaBACE Sequencing ScoreCard offers a time-saving overview of all samples.
* MegaBACE Fragment Profiler allows the user to automate the analysis of di-, tri-, and tetranucleotide repeats of polyploid organisms. User optimized peak filters allow for the automated analysis of atypical peak patterns.
* MegaBACE Genetic Profiler is optimized for diploid dinucleotide repeat analysis.
* MegaBACE Genetic Profiler ScoreCard allows quality comparisons between capillaries and runs.
* MegaBACE SNP Profiler saves editing time by assigning quality scores to each SNP call.
Operating system: Windows 2000
Software: Microsoft Office Pro
Sequencing read length and throughput
Genotyping sizing precision, accuracy, and throughput
The MegaBACE DNA Genotyping System automatically processes 2000 microsatellite genotypes per 96-well run. The total turnaround time per run is less than 2 hours from injection to injection. This corresponds to more than 14 000 genotypes per system per day. For SNP analysis, MegaBACE 1000 can deliver >5 760 SNPs per day.
Minimal sample loading volume of 5 l. The samples are introduced to the system in a 96-well format. Samples can be reinjected up to 10 times.
* Single mode (488 nm) for sequencing
* Dual mode (488 and 532 nm) for genotyping
* Confocal optics with multiple filter sets
Electrophoresis Voltage: Up to 320 V/cm
Chamber Operating Temperature: 27 C to 44 C
Dimensions: 81.2 cm (height) x 103.3 cm (width) x 87.4 cm (depth)
MegaBACE 1000 Instrument, Workstation Computer, Standard PC Monitor, Laser Power Supply and Blower Unit, and accessories (in tub; including Bar-code Reader, interconnect cables, mouse, keyboard, caster wheels with hardware).
AMERSHAM PHARMACIA BIOTECH'S DNA SEQUENCER BECOMES FIRST CAPILLARY-BASED INSTRUMENT TO ACHIEVE VALIDATION WITH PHRED
Sunnyvale, California - Amersham Pharmacia Biotech, the life sciences division of Nycomed Amersham plc (NYSE: NYE; LSE: NAM), announced today that University of Washington will be releasing a new version of PHRED, a crucial sequencing data analysis program used by high-throughput DNA sequencing facilities. The new version of PHRED is re-calibrated for data produced by the MegaBACE 1000 DNA sequencer. It is the only capillary sequencer to be validated with this new version of PHRED.
"We originally developed PHRED for use with older, fluorescent slab gel systems. The data produced by capillary sequencers required a refinement to the program and validation to ensure agreement between predicted quality values and actual quality values in PHRED," said Brent Ewing, PhD at the University of Washington Molecular Biotechnology Department. "We have verified that the quality values assigned by PHRED are accurate for data produced by MegaBACE, and we used over four million base pairs of data supplied by the genome community to calibrate PHRED for it. The community can be confident in using data generated by MegaBACE."
